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Sha Shan is a Vietnamese national who has saved Flash Thompson’s life many times. After a brief stint as a Spider-Man villian called Sister Sun, she gave up crime.
Alistair Smythe is the son of Spencer Smythe: a mad scientist responsible for the creation of Spider-Slayers; robots designed to hunt and kill Spider-man. After his death Alistair carried on his work.
Once Michael Morbius was a world-renowned bio-chemist, dying of an unknown blood disease and desperately searching for a cure. He found that cure, but, in turn, it afflicted him with a curse far worse than any possible disease; the curse of the blood-sucking night-beast; the curse of the Living Vampire.
Secretary to J. Jonah Jameson.
Roderick Kingsley became a worthy heir of the Goblin legacy, creating his own identity, of the Hobgoblin, rather than being just another Green Goblin clone.
Symkarian ruler and leader of a band of mercenaries known as the Wild Pack. More recently she has allied herself with Misty Knight, Paladin and the Heroes for Hire operation.
Marla Jameson is the second wife of J. Jonah Jameson. She was a scientist specializing in robotics and helped create weapons to be used against Spider-Man - specifically the Spider-Slayers. When Smythe came after Jonah and his family, Marla was unfortunately killed by him.
Ned Leeds was a Daily Bugle reporter whom Roderick Kingsley brainwashed into becoming the Hobgoblin until his eventual demise.
Mary Jane Watson is an actress and a major love interest of Peter Parker aka Spider-Man. They have had a long and dramatic on-and-off-again relationship. She's one of the few people who know who he is and still supports him as a close friend and confidante.
Richard Parker is the father of Peter Parker (Spider-Man), the husband of Mary Parker, and the younger brother of Ben Parker. Richard worked for the CIA.
Mary Parker is the mother of Peter Parker (Spider-Man). However, she and her husband, Richard Parker, were killed in a plane accident when Peter was still very young.
A burglar Spider-Man allowed to escape at one point, he would later kill Uncle Ben.
The White Rabbit is a young woman with a dangerous obsession with Lewis Carroll. She is an enemy of Spider-Man.
The father of Dr. Octopus.
A member of the Inheritors, a race of beings that feed on totems, a fusion of humans and animals with powers of both. He is instrumental in Spider-Man's transformation, where he eventually "embraces the Other".
The second and most successful clone of Spider-Man created by the Jackal. A superhero known as the Scarlet Spider, Reilly later replaced Peter Parker for a time as Spider-Man after he found out that all this time he was the original and not Peter. Ben met his ultimate demise in the fight against the Green Goblin which led to Peter becoming Spider-Man once again, after he found out that he is the real Spider-Man after all.
After encountering the Kree hero Captain Marvel, Carol Danvers was accidentally subjected to otherworldly radiation that transformed her into a superhuman warrior. Calling herself Ms. Marvel, she established herself as one of the most powerful and prominent heroes and respected member of the Avengers. She has now adopted the mantle of Captain Marvel for herself.
A retired vaudevilian and love interest to Aunt May. He was also friends with Adrian Toomes, the original Vulture.
Anna May Watson is best friends with May Parker and a paternal aunt to Mary Jane Watson. Once the Watsons were neighbors to the Parkers in Forrest Hills.
Former New York City Police Department officer and Peter Parker's ex-girlfriend. She was briefly transformed by the Goblin Serum, but has since been returned to normal.
The father of the former editor of The Daily Bugle, J. Jonah Jameson, and currently married to Peter Parker's Aunt May.
A journalist who worked at the Daily Bugle along with Peter Parker and after Civil War in Front Line newspaper. She seems to like sarcastic humor and Peter is usually the joke.
Max Modell was the head of Horizon Labs and also the employer and good friend of Peter Parker.
Uatu is a pre-teen genius who works in Horizon Labs.
Bella is a co-worker of Peter Parker at Horizon Labs.
Oksana married Rhino and turned him away from a life of crime. When she was killed by the new Rhino, the original Rhino blamed Spider-Man and returned to a life of crime.
The Kid Who Collects Spider-Man
A co-worker of Peter Parker's at Horizon Labs.
Sajani worked in Horizon Labs and currently, in Parker Industries. She is the creator of the Reverbium.
Editor-in-Chief of the Daily Bugle, and perhaps the best friend to J. Jonah Jameson.
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