At the start of this issue, Peter Parker is standing alone in his apartment, worrying since he might be a clone. After a moment of pondering, he swings off to Curt Conner's laboratory, so try and see if he can test and see if he's a clone. As he does so, a mysterious figure watches him on a video screen, planning revenge against Spider-Man. In the lab, Spider-Man is trying to work out how to he can test if he's a clone or not, when Curt Connors comes in. Spider-Man tells him his problem, and then they start doing tests. After a few hours, they're done, although Connors tells Spider-Man that they won't have the final results for a few hours, and that he should lie down for a while.
Spider-Man wakes up to hear the Vulture outside, taunting him. He worries that if the Vulture were to harm Connors, then he won't know whether he's a clone or not, and so jumps out to attack the Vulture. After a brief scuffle, the Vulture charges into a wall as Spider-Man lands on his back. Spider-Man jumps off just before he hits it, and for some reason the Vulture explodes when he collides with the wall. As Spider-Man tries to work out what happens, he is attacked by the Sandman. Spider-Man manages to drop a water tower on Sandman, but as he does, the Sandman also explodes, just as the Vulture did. By now Spider-Man is really confused, and still worn out from the tests, and so swings back to Connors lab. But on the way, he is attacked by the Kingpin. Spider-Man lands in a few punches, but just like with Sandman and Vulture, Kingpin explodes.
Spider-Man then stumbles through an alley, wondering if it could all be a dream. However, he is unexpectedly approached by Spencer Smythe in his Spider-Slayer, who was the mysterious person watching him before. As Smythe fights Spider-Man, he tells him of how the villains he's fought that night were just robots of his that he made, designed to weaken him both physically and mentally. Spider-Man thinks he's going to die, and realizes that Mary Jane is the girl he loves. He then realizes that since he likes Mary Jane, and not Gwen, he mustn't be the clone, who would have been motivated by Professor Warren to like Gwen. With this knowledge, Spider-Man smashes the Spider-Slayer to bits, not giving up now that he knows who he is.
Spider-Man then goes back to Connors' lab, where he finds that Curt has finished his results. However, he simply throws them away, since he already knows.