I can only list 15 people that Spider-man have defeated. If you think you can list more than 15 people than try it. bet you won't.

Electro.

Mysterio.

Lizard.

Kraven.

Vermin.

Carnage.

Venom.

Rhino.

Iron Man.

Green Goblin.

Hobgoblin.

Menace.

Doctor Octopus (Bleh.)

Kaine.

Jackal.

Morbius.

Molten Man.

Chameleon.

Vulture.

Sandman.

Hydroman.

Keep in mind, that's not listing multiple versions of the same villain, which Spidey has fought a great deal of.

@ryugathedragonemper: Are you serious? You've been reading spider-man comic books for how long? 1 year?

• Beetle
• Beyonder
• The Big Man (Frederick Foswell)
• Big Wheel
• Black Tarantula
• Boomerang
• The Burglar — this unnamed small-time crook played an essential role in Spider-Man's origin. Spider-Man originally intended to use his powers for fame and fortune, but one day allowed a criminal to run past him. Soon after, the same criminal killed Uncle Ben -- and Peter learned that he could have prevented the death by using his powers more responsibly.
• Calypso
• Carnage
• Carrion
• Chameleon
• Demogoblin
• Doctor Octopus (Otto Octavius) aka Master Planner
• Doctor Octopus (Carolyn Trainer) aka Lady Octopus
• Doppelganger
• Electro
• Enforcers
• The Finisher is an assassin employed by the third Red Skull, Albert Malik. He killed Spider-Man's parents. Spider-Man kills him in self defense (Amazing Spider-Man Annual #5) by turning his own missile against him.
• Fortunato
• Fusion
• Green Goblin (Norman Osborn)
• Green Goblin (Harry Osborn)
• Grizzly
• Hammerhead
• Hobgoblin
• Jackal
• Jack O'Lantern and Mad Jack
• Kangaroo
• Kingpin
• Kraven the Hunter
• Living Brain
• The Lizard
• Looter
• Midnight
• Mister Fear
• Morbius the Living Vampire
• Mysterio
• Overdrive
• Rhino
• Ringer
• The Rose (Richard Fisk) aka the Schemer
• Sandman
• Scarecrow
• Scorpia
• Scorpion
• Shocker
• Shriek
• Silvermane
• The Sinister Six, Sinister Seven, and Sinister Twelve, teams of Spider-Man's enemies
• The Sinister Syndicate, another team of Spider-Man's enemies
• Alistair Smythe
• Spencer Smythe
• Speed Demon
• Spider-Slayers (robots)
• The Spot
• Stegron
• Stunner
• Swarm
• Tarantula (Anton Miguel Rodriguez)
• Tinkerer
• Tombstone
• Trapster
• Typeface
• Venom
• Vermin
• Vulture
• Sentry
• White Rabbit
• Will o' the Wisp

http://marvel.wikia.com/Spider-Man_Villains

That's the list that Marvel provides. Pretty sure, it's incomplete. You were saying?

As I said above, the list is actually incomplete (I've checked it).

Titania

The Hood's Gang

Ana Kravinoff

Grim Hunter

Alyosha Kravinoff

Sasha Kravinoff

Captain America

Blackie Drago

Wolverine

Daredevil