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Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
Mary Jane Watson is an actress and a major love interest of Peter Parker aka Spider-Man. They have had a long and dramatic on-and-off-again relationship. She's one of the few people who know who he is and still supports him as a close friend and confidante.
Classmate of Peter Parker at Empire State University
Lance Bannon was a former rival photographer of Peter Parker and boyfriend to Amy Powell. He was later murdered by the man known only as F.A.C.A.D.E.
Captain Universe is the physical manifestation of the Uni-Power. Once it possesses a host, it grants immense power for the purpose of protecting universal balance.
The Tri-Sentinel was originally three separate Project: NIMROD prototypes who were fused into a single entity by Loki's dark magic.
Fellow student and brief girlfriend of Spider-Man
A university professor that taught physics and Peter Parker was one of his student teachers.
In the year 2099, Miguel O'Hara, the head of the genetics department for the Alchemax Corporation becomes the legendary hero know as Spider-Man.
A student who became the costumed hero, the Bluebird.
The amoral industrialist Norman Osborn was transformed by an experimental serum into the maniacal Green Goblin, and has endured over the decades as Spider-Man's most dreaded archenemy. Osborn is known for being the killer of Gwen Stacy, the architect of the Clone Saga, and the one-time director of S.H.I.E.L.D.
Scrier is a godlike being that claims to be the architect of Earth
Part the "popular crowd" along with Flash Thompson, Brian "Tiny" McKeever, Seymour O'Reilly, and Sally Avril who were often picking on Peter Parker.
Hobie Brown is a brilliant inventor whom started out as a criminal known as the Prowler, but soon turned to a more heroic path and became a trusted ally of Spider-Man. He later gave up the Prowler identity and became the Hornet.
Son of Wilson and Vanessa Fisk. Took up the identity of The Rose to become a rival to his father, and later became Blood Rose to act as a vigilante who openly fought against his father.
Silvio Manfredi has always been some kind of villain. His body is almost completly made up of cybernetic parts and he is almost like a human robot.
Puma is a powerful Native-American Werecat. Puma was raised to fight the Beyonder and became an ally to Spider-Man.
Molten Man is the stepbrother of Liz Allen. He has had several run-ins with Spider-Man. He was recently cured of his condition.
The Jackal is an enemy of Spider-Man who played a big role in both of the Clone Sagas. He has since seemingly returned to infest Manhattan with spider-powers for his own sinister ends, organizing test subjects into a spider-powered army.
Kaine was the first clone of Spider-Man created by the Jackal. He was suffering from clone degeneration and became an assassin and spent years tormenting his clone brother, Ben Reilly. He has since taken up the mantle his now dead brother as the new Scarlet Spider.
Police officer Jake Gallows follows in the bloody, bullet-riddled footsteps of Frank Castle, bringing his one-man war on crime into the year 2099.
Symkarian ruler and leader of a band of mercenaries known as the Wild Pack. More recently she has allied herself with Misty Knight, Paladin and the Heroes for Hire operation.
Ravage 2099 is a hero of Earth-928, he is a mutate, whose mutation is constantly changing and evolving. He went from the head of ECO to the leader of Hellrock in his time.
Secretary to J. Jonah Jameson.
A small time criminal that worked his way up to having his own crime family within the Maggia.
Howard is a cigar-smoking anthropomorphic duck who lived in New Stork City on the planet Duckworld (in a parallel dimension). He was transported to and became trapped on our world, where he became close friends with Beverly Switzler. Most people of Earth simply do not believe that he is a talking duck.
Once Michael Morbius was a world-renowned bio-chemist, dying of an unknown blood disease and desperately searching for a cure. He found that cure, but, in turn, it afflicted him with a curse far worse than any possible disease; the curse of the blood-sucking night-beast; the curse of the Living Vampire.
Alistair Smythe is the son of Spencer Smythe: a mad scientist responsible for the creation of Spider-Slayers; robots designed to hunt and kill Spider-man. After his death Alistair carried on his work.
The second and most successful clone of Spider-Man created by the Jackal. A superhero known as the Scarlet Spider, Reilly later replaced Peter Parker for a time as Spider-Man after he found out that all this time he was the original and not Peter. Ben met his ultimate demise in the fight against the Green Goblin which led to Peter becoming Spider-Man once again, after he found out that he is the real Spider-Man after all.
MacDonald "Mac" Gargan was a failing private investigator that was turned into the Scorpion with financial help from J. Jonah Jameson. He became a host of the Venom symbiote and was part of Norman Osborn's Dark Avengers. After the US Government took the symbiote, he became The Scorpion once again.
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