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Gwen Stacy was the first true love of Peter Parker. She's best known for her tragic death by being thrown off from the Brooklyn Bridge by the Green Goblin. The Earth-65 version is Spider-Gwen who is a major player in the Spider family.
Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
Flash frequently bullied Peter Parker but was actually Spider-Man's greatest fan. In time, Flash befriended Peter, became a decorated war hero, and eventually joined the Marvel superheroes as a symbiote host, first with Venom and then with Anti-Venom.
Mary Jane Watson is an actress and a major love interest of Peter Parker aka Spider-Man. They have had a long and dramatic on-and-off-again relationship. She's one of the few people who know who he is and still supports him as a close friend and confidante.
Harry Osborn is the best friend of Peter Parker, the Amazing Spider-Man. His father is Norman Osborn, a global industrialist and billionaire.
Peter Parker's aunt-by-marriage and one of the most important people in his life.
A famous big game hunter, who became a mercenary and later, developed an intense obsession with restoring honor to his name. Kraven has been known as a major threat for Spider-Man and leaving a legacy during his demise.
During an experiment, Dr. Curtis Connors accidentally turned himself into a ferocious reptilian monster who seeks to turn humans into lizards like himself. The Lizard is one of Spider-Man’s oldest and deadliest foes while ironically, his alter ego is a close friend and former teacher of Spidey’s alter ego, Peter Parker.
Swindled by his business partner out of his equal share of the profits from their company, Adrian Toomes set out for revenge by using a flying harness of his own design. He has however, met defeat several times at the hands of his arch-nemesis, The Amazing Spider-Man.
The second Vulture, who took the original Vulture's wings after tricking him into telling him their location. He was defeated by Spider-Man quite easily.
The amoral industrialist Norman Osborn was transformed by an experimental serum into the maniacal Green Goblin, and has endured over the decades as Spider-Man's most dreaded archenemy. Osborn is known for being the killer of Gwen Stacy, the architect of the Clone Saga, and the one-time director of S.H.I.E.L.D.
Betty Brant started as a personal secretary at the Daily Bugle and later became a top reporter. Betty is also one of the many love interests of Peter Parker and ex-girlfriend of Flash Thompson
A career criminal, the man known as Rhino has incredible strength and durability. A long-time enemy of Spider-Man and the Incredible Hulk, Rhino isn't particularly malicious, and has tried on various occasions to leave behind his life of crime, but always seems to slide back into old habits.
Editor-in-Chief of the Daily Bugle, and perhaps the best friend to J. Jonah Jameson.
J. Jonah Jameson is the former employer of Peter B. Parker and remains an aggravate of Spider-Man. He was once the chief editor and publisher of the Daily Bugle, a New York newspaper, and the mayor of New York before his resignation amidst scandal resulting from Superior Spider Man and the Goblin Nation. He now is a host of a show on the F.A.C.T. News Channel
MacDonald "Mac" Gargan was a failing private investigator that was turned into the Scorpion with financial help from J. Jonah Jameson. He became a host of the Venom symbiote and was part of Norman Osborn's Dark Avengers. After the US Government took the symbiote, he became The Scorpion once again.
A former investigative journalist for the Daily Bugle and current owner of the newspaper Front Line who uses his friendships with Daredevil and Spider-Man to get information on criminals.
William ''Billy'' Connors is the son of Martha and Curt Connors (the Lizard).
An American comedian and TV host, Jay Leno (born on April 28, 1950) is known for his large chin and his collection of classic cars and motorcycles. He was the host of NBC's classic late-night talk show The Tonight Show from 1992 to 2014, with a brief break from 2009 to 2010 when he hosted the primetime talk show The Jay Leno Show. After retiring from The Tonight Show, Leno began hosting the web series"Jay Leno's Garage" on NBC.com, which was later expanded into a TV show on CNBC.
The Jackal is an enemy of Spider-Man who played a big role in both of the Clone Sagas. He has since seemingly returned to infest Manhattan with spider-powers for his own sinister ends, organizing test subjects into a spider-powered army.
Martha is the wife of Curt Connors, mother of Billy Connors.
Anna May Watson is best friends with May Parker and a paternal aunt to Mary Jane Watson. Once the Watsons were neighbors to the Parkers in Forrest Hills.
Doctor Octopus is a supervillain originating from Marvel Comics. A former nuclear physicist and atomic researcher, Otto Octavius' life changed forever when one of his experiments went horribly wrong involving his four mechanical appendages used to assist him in his experiments. From then on, Octavius soon after his initial encounter has become one of the most dangerous supervillains in the Marvel Universe.
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