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Peter Parker was bitten by a radioactive spider as a teenager, granting him spider-like powers. After the death of his Uncle Ben, Peter learned that "with great power, comes great responsibility." Swearing to always protect the innocent from harm, Peter Parker became Spider-Man.
Editor-in-Chief of the Daily Bugle, and perhaps the best friend to J. Jonah Jameson.
Harry Osborn is the best friend of Peter Parker, the Amazing Spider-Man. His father is Norman Osborn, a global industrialist and billionaire.
Betty Brant started as a personal secretary at the Daily Bugle and later became a top reporter. Betty is also one of the many love interests of Peter Parker and ex-girlfriend of Flash Thompson
An enemy of Spiderman.
The former CEO of Damage Control who sold Nitro the MGH he was hopped up on when he exploded in Stamford, thus igniting Marvel's Civil War.
Jackpot is a new registered hero with The Initiative who fights in New York City. There have been two people to take the Jackpot identity, Alana Jobson who died, and first and current heir Sara Ehret.
Former New York City Police Department officer and Peter Parker's ex-girlfriend. She was briefly transformed by the Goblin Serum, but has since been returned to normal.
Harry Osborn's fiance who absorbed a unique variation of the Goblin Serum that allows her to shapeshift into a goblin. After losing those powers, she assumed the persona of Queen Cat.
Rumor monger and scandal sheet printer, Dexter Bennett became the latest owner of the Daily Bugle after a hostile buyout of long-time owner J. Jonah Jameson.
A police officer in New York. Doesn't have faith that Spider-Man is a good guy.
Working for Walter Declun, Blindside's suit secretes a neurotoxin that temporarily blinds whoever he touches.
Skrull that impersonated Spider-Man during Secret Invasion.
Alana Jobson was the second person to operate as the Initiative-registered superhero Jackpot (although she was the first person portrayed as Jackpot on-panel). She was a social worker who took over the Jackpot persona from Sara Ehret, using mutant growth hormone drugs to gain powers while in costume. She died in the line of duty.
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