Punch and Jewelee were working as puppeteers and moonlighting as thieves at the Coney Island fair grounds when they discovered a strange, antique-looking chest that had floated onto the beach. Inside, they found several alien devices: flying boots, sting strings and hypno gems. There was also a mind recorder device which explained how to use the other devices.
They decided to use these devices and model themselves on their puppet characters, Punch and Judy, with Jewelee altering the name to fit in with the hypno-gems. The pair charmed real estate agents and construction workers into building them a laboratory under Coney Island. With the mind recording device, they planned to kidnap several prominent scientists, drain their minds and sell the information to the highest bidder. The plan backfired when they kidnapped Alec Rios, who was working along side Nathaniel Adam (Captain Atom) and Eve Eden (Nightshade).
The two heroes teamed up to thwart the plans of Punch and Jewelee, but the villains escaped to fight another day.
Amanda Waller recruited Punch and Jewelee for a mission with the Suicide Squad. The duo lacked focus and had an odd predisposition for violence. Waller later decided to keep Punch and Jewelee under observation as active squad members, despite Bronze Tiger feeling that they were a liability to the team. After Jewelee learned she was pregnant, she and Punch left the Suicide Squad and tried to settle down in Middle America. They were later seen in Washington D.C, attempting to steal an experimental force-field vest to protect their baby (later confirmed to be a boy).
After Infinite Crisis Punch, and Jewelee were seen stealing from an art museum. They are stopped by Manhunter, and Obsidian. During a mission with a reformed Suicide Squad, Punch is fatally shot by soldiers while trying to protect Jewelee, leaving her to raise their son alone.
Jewelee was one of the villains sent to planet Salvation (from the Salvation Run story arc) , where she primarily served as the Joker's arm candy.
In other media
She was seen along side Punch in Batman the Brave and the Bold. They are defeated by Batman and as they are carried away Booster Gold approaches them to be his enemies in the future.