Most spine chilling moments of anime

What moments in anime sent massive chills down your spine? From either the creepiness or the sadness or the sheer epicness of a scene. Make your choice.

Here is my example:

Huges death in Full Metal Alchemist (everyone, his daughter/wife crying).

the funeral. Roy crying "what a terrible day to rain"

it kills me

Pikachu's goodbye

I think my definition of "chills/shivers down your spine" is completely different from everyone elses' in this thread.

Anything from Higurashi.

Every moment in Attack on Titan

So many f_cked up scenes flashed through my mind after reading the OP that I think I should watch a few episodes of Adventure Time to regain normalcy.

Anything involving incest. The unfathomable horror!

1. Light revealing he's Kira

2. Lelouch turning evil in Code Geass

3. Any scene with Orochimaru in Naruto

4. Goku sacrificing his life to beat Cell

5. Huges death in FMA