According to the following, Jessica Drew aka Ultimate Spider-woman has been confirmed to be a lesbian
Comic Book Resources listed on there list of 63 LGBT Marvel/DC characters
Marvel Comics does have an array of homosexual or bisexual characters. Many, like the only recently outed Iceman, belong to the X-Men and hence Fox’s movies. Others are young or from the Ultimate Universe, like the Ultimate Spider-Woman, Jessica Drew, or Young Avengers Hulking and Wiccan. None are top tier when it comes to marketability.
I suppose we at least I should have seen it coming, or I did in All-New Ultimates and just forgot.
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