Hey

Are pretty much all of the Villains in the New 52 volumes? I have tried to read lists and been given instructions to which novels to read but I think the New 52 will be the best way forward? I don't want to miss anything or miss the villains coming into them.

new 52 is a reboot and in time to time there will be villains who come and villains who get lost in time... dr death was lost and rebooted in vol 5

I'm not quite sure what you're asking

The new 52, like @arkhamace said, is a reboot. In a reboot you are not obliged to re-introduce all the previous characters/vilains. They have for some, but not all of them. But they also have introduced new characters/vilains.

And for the answer to "the New 52 will be the best way forward?", it's a way, the best I don't know. I also think that the new 52 have a lot of titles. It's hard to follow every things.

Then to miss nothing : look at every tpb that DC publishes and try to get them as they come out. You should read in order of publication.

If you want to focus only on Batman, the titles/books to read are : The Batman - Batman, the dark knight (it focuses on stories where batman is acting alone) - Detective Comics - Batman crossover titles (like Night of the owl) - Batman min-series (like Batman eternal).

If you're asking have we seen all the villains Batman's ever had, or ever a majority of them, within the New 52 so far the answer is a definite no. However, we have seen many of the most well known villains, such as the Joker, Mr. Freeze, Two Face, the Riddler, etc. And it's just been with varying degrees of focus.

The New 52 is a sure way to get involved and understand what's going on without too much issue, but it's still itself fairly new and hasn't gone into much depth for most of the villains shown, so if you're looking for that you'd probably be better off reading the New 52 and picking up some older volumes as well.